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| Registros recuperados : 5 | |
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Registro Completo
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Biblioteca(s): |
Embrapa Milho e Sorgo. |
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Data corrente: |
17/11/1999 |
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Data da última atualização: |
23/08/2020 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
CANCADO, G. M. A.; LOGUERCIO, L. L.; MARTINS, P. R.; PARENTONI, S. N.; PAIVA, E.; BOREM, A.; LOPES, M. A. |
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Afiliação: |
EMBRAPA/CNPMS; SIDNEY NETTO PARENTONI, CNPMS. |
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Título: |
Hematoxylin staining as a phenotypic index for aluminum tolerance selection in tropical maize (Zea mays L.). |
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Ano de publicação: |
1999 |
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Fonte/Imprenta: |
Theoretical and Applied Genetics, New York, v. 99, n. 5, p. 747-754, 1999. |
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Idioma: |
Inglês |
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Conteúdo: |
Hematoxylin staining is an early indicator of aluminum (Al) toxicity effects on the apices of young, developing roots grown in nutrient solution. In this work, the potential of this technique as a reliable and reproducible phenotypic index for Al tolerance in tropical maize genotypes was assessed, with its performance systematically compared to two other parameters widely used in breeding programs - relative seminal-roots length (RSRL) and net seminal-root length (NSRL). Seeding roots from contrasting genotypes for Al sensitivity stained remarkably different after 24- and 48-h and 7-day exposures to 222 uM Al in nutrient solution, with the Al-dye complex being detected in both the outer (epidermis) and inner (cortex) portions of the roots from the sensitive cultivar. Hemotoxylin staining was compared to the RSRL and NSRL parameters using 20 families from the third generation of selfing (S3) following the cross between two contrasting inbred lines that had been previously classified by the RSRL index in an independent procedure. The coloration technique showed the highest capacity to discriminate among tolerant and sensitive genotypes and displayed significant correlation coefficient to the other two indexes. Evaluation of the results from diallel crosses involving nine inbred lines proved that hematoxylin staining was also particularly adequate for identifying expressive hybrid vigor, as demonstrated by the general (GCA) and specific (SCA) combining ability estimates obtained by using the three indexex simultaneously. Hence, hematoxylin staining of Al-stressed root apices appears to be a powerful tool to assist in Al-tolerance selection in tropical maize breeding programs. MenosHematoxylin staining is an early indicator of aluminum (Al) toxicity effects on the apices of young, developing roots grown in nutrient solution. In this work, the potential of this technique as a reliable and reproducible phenotypic index for Al tolerance in tropical maize genotypes was assessed, with its performance systematically compared to two other parameters widely used in breeding programs - relative seminal-roots length (RSRL) and net seminal-root length (NSRL). Seeding roots from contrasting genotypes for Al sensitivity stained remarkably different after 24- and 48-h and 7-day exposures to 222 uM Al in nutrient solution, with the Al-dye complex being detected in both the outer (epidermis) and inner (cortex) portions of the roots from the sensitive cultivar. Hemotoxylin staining was compared to the RSRL and NSRL parameters using 20 families from the third generation of selfing (S3) following the cross between two contrasting inbred lines that had been previously classified by the RSRL index in an independent procedure. The coloration technique showed the highest capacity to discriminate among tolerant and sensitive genotypes and displayed significant correlation coefficient to the other two indexes. Evaluation of the results from diallel crosses involving nine inbred lines proved that hematoxylin staining was also particularly adequate for identifying expressive hybrid vigor, as demonstrated by the general (GCA) and specific (SCA) combining ability estimates obtaine... Mostrar Tudo |
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Thesagro: |
Aluminio. |
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Categoria do assunto: |
G Melhoramento Genético |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/40394/1/Hematoxylin-staining.pdf
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Marc: |
LEADER 02337naa a2200205 a 4500
001 1482859
005 2020-08-23
008 1999 bl uuuu u00u1 u #d
100 1 $aCANCADO, G. M. A.
245 $aHematoxylin staining as a phenotypic index for aluminum tolerance selection in tropical maize (Zea mays L.).$h[electronic resource]
260 $c1999
520 $aHematoxylin staining is an early indicator of aluminum (Al) toxicity effects on the apices of young, developing roots grown in nutrient solution. In this work, the potential of this technique as a reliable and reproducible phenotypic index for Al tolerance in tropical maize genotypes was assessed, with its performance systematically compared to two other parameters widely used in breeding programs - relative seminal-roots length (RSRL) and net seminal-root length (NSRL). Seeding roots from contrasting genotypes for Al sensitivity stained remarkably different after 24- and 48-h and 7-day exposures to 222 uM Al in nutrient solution, with the Al-dye complex being detected in both the outer (epidermis) and inner (cortex) portions of the roots from the sensitive cultivar. Hemotoxylin staining was compared to the RSRL and NSRL parameters using 20 families from the third generation of selfing (S3) following the cross between two contrasting inbred lines that had been previously classified by the RSRL index in an independent procedure. The coloration technique showed the highest capacity to discriminate among tolerant and sensitive genotypes and displayed significant correlation coefficient to the other two indexes. Evaluation of the results from diallel crosses involving nine inbred lines proved that hematoxylin staining was also particularly adequate for identifying expressive hybrid vigor, as demonstrated by the general (GCA) and specific (SCA) combining ability estimates obtained by using the three indexex simultaneously. Hence, hematoxylin staining of Al-stressed root apices appears to be a powerful tool to assist in Al-tolerance selection in tropical maize breeding programs.
650 $aAluminio
700 1 $aLOGUERCIO, L. L.
700 1 $aMARTINS, P. R.
700 1 $aPARENTONI, S. N.
700 1 $aPAIVA, E.
700 1 $aBOREM, A.
700 1 $aLOPES, M. A.
773 $tTheoretical and Applied Genetics, New York$gv. 99, n. 5, p. 747-754, 1999.
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Registro original: |
Embrapa Milho e Sorgo (CNPMS) |
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